By Sara Bobone
In her thesis, Sara Bobone outlines spectroscopic experiences of antimicrobial peptides (AMPs) that are promising lead compounds for medicinal drugs used to struggle multidrug resistant micro organism. Bobone exhibits that AMPs have interaction with liposomes and he or she clarifies the constitution of pores shaped by means of the sort of molecules. those effects aid us to appreciate how AMPs are selective for bacterial membranes and the way their task should be finely tuned by means of editing their series. Findings which clear up a number of conundrums debated within the literature for years. furthermore, Bobone makes use of liposomes as nanotemplates for the photopolymerization of hydrogels - exploiting the self- meeting houses of phospholipids. Bobone was once capable of capture an enzyme utilizing nanometeric debris, whereas nonetheless permitting its task by means of the diffusion of substrates and items during the community of the polymer. The leading edge nano units defined during this thesis might remedy some of the hurdles nonetheless hampering the healing program of protein-based drugs.
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Additional resources for Peptide and Protein Interaction with Membrane Systems: Applications to Antimicrobial Therapy and Protein Drug Delivery
Spectroscopic grade methanol, ethanol and chloroform were purchased from Carlo Erba Reagenti (Milano, Italy). Carboxyfluorescein (CF), Triton-X 100, Sephadex-G50 and 1,6-Diphenyl-1,3,5-hexatriene (DPH) were purchased from a Sigma Aldrich (Germany). The fluorophore sodium-binding benzofuran isophthalate (SBFI) was purchased from Invitrogen (Eugene, USA). 2 Peptide Synthesis All peptides used for this study were prepared by the standard Fmoc-based solidphase method . Pep-1, Pep-1-K, P5 and P5 analogues were synthesized by the Research Center for Proteinous Materials (Chosun University, Korea).
7 Membrane-Perturbing Activity Experiments 23 Peptide effects on membrane order and dynamics were determined by measuring the fluorescence anisotropy of 100 nm DPH labeled-DMPC liposomes, at a kexc. = 350 nm and kem. = 450 nm, at different temperatures or at different peptide concentrations. 8 Water-Membrane Partition Experiments For water-membrane partition experiments the Trp fluorescence signal of each peptide was followed titrating 1 lM peptide solutions with increasing amounts of 100 nm ePC/ePG or ePC/cholesterol liposomes.
In this case, a perfect amphipathic helix was obtained (Fig. 19). 3. 5 Antimicrobial and Hemolytic Activity The antimicrobial activity of P5 and its analogues was tested against different Gram-positive and Gram-negative bacteria. The bactericidal activity is expressed as minimum inhibitory concentration, or MIC, defined as ‘‘the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight incubation’’ . All analogues featured a good antimicrobial activity, even though the Pro displacement caused a slight increase in MIC values, that are shown in Fig.
Peptide and Protein Interaction with Membrane Systems: Applications to Antimicrobial Therapy and Protein Drug Delivery by Sara Bobone