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By Bernard Pullman

ISBN-10: 0125669607

ISBN-13: 9780125669603

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Extra resources for Frontiers in Physicochemical Biology

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X , € 5 n m ; • • , € 2 6 o n m , ' Δ -· - Δ , €265nmReprinted with permission from Pless and Ts'o (1977), Biochemistry 16, 1239. Copyright by the American Chemical Society. 001 Μ sodium cacodylate at 20°C (Fig. 21b). The low molecular weight peak makes up 47% of the total d ( T E t ) and is termed d ( T E t ) (7°-20°C), that is, the d(T -Et) fraction that comigrates with (dA) at 7°C but does not comigrate at 20°C. Rechromatography of the high molecular weight fraction on Sephadex G-50 at 30°C (Fig.

A mixing curve for this system is shown in Fig. 20. Since the sample of d(T · Et) should consist of a large number of diastereoisomers, the annealing curve observed might result from the superimposition of a multitude of oligomer-polymer transitions with different stabilities. To address this possibility, an attempt was made to fractionate the octamer triester according to its ability to comigrate with the deoxyadenylate polymer on a Sephadex G-50 column at different temperatures. 9). 9, was incubated at 4°C for 1 hour and then chromatographed on a Sephadex G-50 column (137 ml) equilibrated at 7°C.

In the present investigation, a novel approach to this problem was adopted: transformation of one negatively charged strand [d(T ) in this case] into a nonionic strand. 4 kcal/mole of base pairs between duplex formation from two charged strands [(dA) + d(T ), ΔΗ = - 6 . 4 kcal].

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Frontiers in Physicochemical Biology by Bernard Pullman


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